5280 Drug Testing locally serves Denver, Littleton, Colorado Springs, Ft. Collins, Loveland, Greeley, Grand Junction and all points in between…

Our Locations:

5280 Drug Testing locations offer an executive environment for employees do their pre-employment, post-accident, and random drug and alcohol testing.  We appreciate and understand that this part of the employee screening process can be very uncomfortable, therefore we go above and beyond to provide the most pleasant and dignified experience possible. To see photos of our Greenwood Village office, click here.

We offer a safe environment that is family friendly. Rest assured that whether you are coming in for pre-employment or for private testing, your children are welcome.  While we offer services for probation, DUI and other court related cases, we do not offer services to violent criminal or sex offenders.

**Should your company/ employee be located outside of one of our service areas, we have an extensive 25,000+ collection site network that we utilize to provide the most convenient testing location.  Whether you’re close to home or across the country, we’ve got you covered.

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One convenient Colorado location to serve you/ State & Nationwide Testing Available with 25,000+ collection site network.



Walk in’s Welcome


Monday – Friday 9am-530pm
Saturday 9am-Noon

5280 Drug Testing is located in the Creekside II Office Park, just 3/4 mile off I-25 near the intersection of S. Quebec and Orchard St. at:
6059 S. Quebec St., #100
Greenwood Village, CO 80111
720-476-4970 phone
888-620-9502 fax


No matter where you are located in the United States, 5280 Drug Testing can assist you with all of your drug and alcohol testing needs. Call 720-476-4970 or email [email protected] to find a location.

National Coverage with access to over 25,000 collection sites